PROSS (Protein Repair One-Stop Shop) [required data 0% complete] Search words in help
If you are using PROSS for the first time, please read our About page.

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Examples and Frequently Asked Questions
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Algorithm and processing: Adi Goldenzweig
Server: Jaime Prilusky
Overview: Sarel Fleishman
PROSS is free to academic users.
Commercial users, please email and cc
Please cite:

Goldenzweig, A. et al. Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability. Mol. Cell 2016, 63 (2), 337346.
PMID: 27425410 PMCID: PMC4961223 DOI: 10.1016/j.molcel.2016.06.012 PDF HTML

For more about the rationale behind PROSS, checkout our review in Ann Rev Biochem:

Goldenzweig, A.; Fleishman, S. Principles of Protein Stability and Their Application in Computational Design. Annu. Rev. Biochem. 2018.
PMID: 29401000

More studies using PROSS:

  1. Campeotto, I.; Goldenzweig, A.; Davey, J.; Barfod, L.; Marshall, J. M.; Silk, S. E.; Wright, K. E.; Draper, S. J.; Higgins, M. K.; Fleishman, S. J. One-Step Design of a Stable Variant of the Malaria Invasion Protein RH5 for Use as a Vaccine Immunogen. Proc. Natl. Acad. Sci. U. S. A. 2017, 114 (5), 9981002.
    PMID: 28096331 DOI:

  2. Brazzolotto, X.; Igert, A.; Guillon, V.; Santoni, G.; Nachon, F. Bacterial Expression of Human Butyrylcholinesterase as a Tool for Nerve Agent Bioscavengers Development. Molecules 2017, 22 (11).
    PMID: 29077024

  3. Despotović, D.; Brandis, A.; Savidor, A.; Levin, Y.; Fumagalli, L.; Tawfik, D. S. Diadenosine Tetraphosphate (Ap4A) - an E. Coli Alarmone or a Damage Metabolite? FEBS J. 2017, 284 (14), 21942215.
    PMID: 28516732

  4. Bandyopadhyay, B.; Goldenzweig, A.; Unger, T.; Adato, O.; Fleishman, S. J.; Unger, R.; Horovitz, A. Local Energetic Frustration Affects the Dependence of Green Fluorescent Protein Folding on the Chaperonin GroEL. J. Biol. Chem. 2017, 292(50):20583-20591.
    PMID: 29066625

  5. Goldsmith, M.; Aggarwal, N.; Ashani, Y.; Jubran, H.; Greisen, P. J.; Ovchinnikov, S.; Leader, H.; Baker, D.; Sussman, J. L.; Goldenzweig, A.; et al. Overcoming an Optimization Plateau in the Directed Evolution of Highly Efficient Nerve Agent Bioscavengers. Protein Eng. Des. Sel. 2017, 30 (4), 333345
    PMID: 28159998

  6. Buldun, C. M.; Jean, J. X.; Bedford, M. R.; Howarth, M. SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation. J. Am. Chem. Soc. 2018, 140 (8), 30083018.
    PMID: 29402082